Realizing the Promise of RNAi High Throughput Screening

Realizing the promise of RNAi high throughput screening.

Recently reporting in Nature, Collinet et al. describes the application of quantitative multiparametric methods to a genome-wide RNAi screen for regulators of endocytosis. The study illustrates the power of this approach beyond the identification of new endocytic components to providing insights into the design principles of the endocytic system.

High-throughput RNAi screening reveals novel regulators of telomerase.

Telomerase is considered an attractive anticancer target on the basis of its common and specific activation in most human cancers. While direct telomerase inhibition is being explored as a therapeutic strategy, alternative strategies to target regulators of telomerase that could disrupt telomere maintenance and cancer cell proliferation are not yet available. Here, we report the findings of a h...

SbacHTS: Spatial background noise correction for High-Throughput RNAi Screening

MOTIVATION High-throughput cell-based phenotypic screening has become an increasingly important technology for discovering new drug targets and assigning gene functions. Such experiments use hundreds of 96-well or 384-well plates, to cover whole-genome RNAi collections and/or chemical compound files, and often collect measurements that are sensitive to spatial background noise whose patterns ca...

StemCONN: Realizing the Promise

On April 3, 2013, the fourth biennial StemCONN conference took place at the Omni Hotel at Yale in New Haven, Connecticut. This conference featured talks by scientists from across the country who are currently at the forefront of stem cell research, as well as talks by Edison Liu, President and CEO of the Jackson Laboratory, and Jonathan Rotherberg, PhD, a Yale alumnus and Ion Torrent Systems Fo...

Sustaining and realizing the promise of telemedicine.